Establishment and application of TaqMan PCR for detection of genetically modified soybean

Objective To establish method of TaqMan PCR for detection of genetically modified soybean,and apply it to the market selling GMO's quantitation.Methods RoundUp ReadyTM of genetically modified soybean(Fluka) of 0,0.1%,0.5%,2.0% and 5.0% were used as standard substance.By using specific TaqMan probe sequence 5′(FAM)-cccactatccttcgcaagaccct-3′(TAMRA) and primer sequences F: 5′-tgatgtgatatctccactgacg-3′,R: 5′-tgtatcccttgagccatgttgt-3′ to amplify the exogenous gene CP4EPSPS,we established a standard curve for quantitative detection of genetically modified soybean.Twelve soybean products selling in the market were quantitatively detected.Results The method of TaqMan PCR for detection of genetically modified soybean was successfully established.The equation of standard curve was Y=-2.819 4X+ 27.185 5 and relative coefficiency R2 was 0.999 4.The contents of 5 kinds of genetically modified foods selling in the market were 2.46%、6.26%、13.95%、0.48%、0.66%,respectively.Conclusion This method could quantitative detect genetically modified soybean rapidly,and could be used to analysis the content of genetically modified foods selling in the market.