Study on Ability of Spermatophore Regeneration in Marsupenaeus japonicus

This research,using an integrated method of Extrusion-clip method,experimental ecology,mariculture and individual markings,was aimed at an investigation into the regularity of spermatophore regeneration of male shrimps,Marsupenaeus japonicus.The main results showed that:under the conditions of water temperature(21±2)℃,salinity 32±1,and pH 8.1±0.2,the spermatophore of the male broodstock had 3times multi formation with an average of 2times in one molt cycle;the interval is ranged from 5days to 7days,different batches had no significant differences(p0.05);different batches of regenerated spermatophore had a downward trend in weight and sperm fertilization rate,the difference was notable(p0.05);but sperm count in different batches of regenerated spermatophore is ascent,moreover in the same batch of regenerated spermatophore sperm count had a thereabouts trend.The distribution of individuals with various regeneration ability in the population of male broodstock:all of the broodstock could generate one time,87.5%two times,80.6%three times and 44.4%four times.From the research,we can summarize that there are no significant differences between the size of the shrimps,M.japonicas and the quality of spermatophore.