Immobilization of Fenpropathrin Degradating-enzyme by Ca-alginate

Fenpropathrin degradating-enzyme was immobilized by Caalginate.And degradative characteristics of the immobilized enzyme were determined in the laboratory.Conditions for immobilization of fenpropathrin degradating-enzyme were with alginate:20 g·L~(-1),protein:1.0 g·L~(-1),time:16 h and diameter of enzyme particle 4 mm.Immobilized enzyme for fenpropathrin degradation was stable in pH range pH 5.0 to 10.0,temperature range 25℃ to 50℃,superior than dissociated enzyme.With Lineweaver-Burk method,immobilized enzyme had Km=298.13 nmol·ml~(-1),V_(max)=43.48 nmol·ml~(-1)·min~(-1).Immobilized enzyme vigor was 80% of dissociated enzyme in optimal conditions.Immobilized enzyme could be used 3 times continually.