Objective: To compare the concentrations of ferulic acid and paeoniflorin in Buyanghuanwu decoction with different preparation's time.Methods:The concentrations of ferulic acid and paeoniflorin in Buyanghuanwu decoction with different preparation's time(10~120min) were determined by HPLC respectively.The data from the experiments were treated statistically.Results: It was showed concentrations of ferulic acid and paeoniflorin in Buyanghuanwu decoction were influenced significantly by the preparation's time(P0.05).Conclusion: This study provided a scientific basis to study the preparation's time of formula.