Effects of 2,3,5 4'-tetrahydroxystilbene-2-O-β-D-glucoside on Nitric Oxide Synthase and Gene Expression in Endothelial Cells of Human Umbilical Vein

Objective To investigate the effects of 2,3,5 4'-tetrahydroxystilbene-2-O-β-D-glucoside(THSG) on content of nitric oxide(NO) and activity of endothelial nitric oxide synthase(eNOS) and the mechanisms.Methods The human umbilical vein endothelial cells(HUVECs) were cultured and divided into four groups:the control,THSG(1,10,100 μmol·L-1)groups.Spectrophotometry endothelium assay was used to measure NO and activity of eNOS.RT-PCR was used to analyze the eNOS mRNA expression and Western Blot was used to analyze eNOS protein expression level in HUVECs.Results THSG significantly increased the production of NO,activated NOS and up-regulated expression of eNOS mRNA and protein in a concentration-dependent manner compared with the control group.Conclusion THSG raises expression of eNOS mRNA and protein,which may result in activating NO production and relaxing blood vessels.