Analysis on p53 gene exon 5 mutations in esophageal cancer

Objective To analyze p 53 gene exon 5 mutations in esophageal cancer (EC) specimens in Xi'an city and Linzhou city.Methods Polymerase chain reaction (PCR) direct sequencing technique was used to detect the alteration of p 53 gene exon 5 for EC specimens from Xi'an city and Linzhou city.Results Mutation rate of p 53 gene exon 5 mutation in Xi'an and Linzhou EC specimens were 14 3%(6/42) and 18 6%(9/43) respectively, which were not significantly different ( P 0 05).Point mutation was the main mutation type.In Xi'an EC specimens, there were six mutation sites including 4-point mutation and 2 base deletions.In Linzhou EC specimens,there were 10 mutation sites,including 9-point mutation and 1 insert mutation.In Xi'an p 53 gene exon 5 mutations were in 126 to 128 bases (4/6),while in Linzhou EC specimens only two had mutations in thiese sites.But these distributions of mutation sites were not significantly different ( P 0 05).Conclusion The p 53 gene exon 5 mutation sites in Xi'an were relatively concentrated;in Linzhou were relatively scattered in different codes,which might be owing to the great variety of risk factors in this district.