The objects of glycome and glycomics, the differences between them, as well as the strategies and methods in this field, are discussed in this paper. Some technologies, such as mass spectroscopy,affinity chromatography, capillary electrophoresis, microarray and microfluid have become useful tools in glycome and glycomics. In glcomics, glycotransferases and carbohydrate-binding proteins are two important kinds of proteins, so glycomics is closely connected with proteomics and genomics.