化学
四极飞行时间
质谱法
分光计
串联
四极
串联质谱法
功勋
蛋白质组
四极杆质量分析仪
三级四极质谱仪
分析化学(期刊)
色谱法
航空航天工程
选择性反应监测
光学
物理
生物化学
工程类
原子物理学
作者
Genna Andrews,Brigitte Simons,J. Bryce Young,Adam M. Hawkridge,David C. Muddiman
摘要
The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. Employing a Saccharomyces cerevisiae tryptic digest, careful consideration of several performance features demonstrated that the speed of the TripleTOF contributed most to the resultant data. The TripleTOF system was operated with 8, 20, and 50 MS/MS events in an effort to compare with other MS technologies and to demonstrate the abilities of the instrument platform.
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