Determination of baicalin in its self-microemulsion by HPLC

Objective To establish the determination method of baicalin in its self-microemulsion. Methods HPLC was used with the mobile phase consisted of methanol-water-phosphate( 55 ∶ 45 ∶ 0. 04). The UV detection wavelength was set at 277 nm. The flow rate was 1. 0 m L / min. Results Baicalin was linear in the range of 1. 0-20. 0 μg / m L with the equation of A = 4. 846 9 × 104ρ-5. 126 2 × 104,r = 0. 999 5,and the average recovery of baicalin at high,middle and low concentrations were 100. 4%,98. 60% and 98. 97%,respectively,with RSD of 1. 8%,2. 4% and 3. 3%( n = 3). The concentrations of baicalin in 3 batches were34. 25,33. 95 and 35. 60 μg / m L. Conclusion This method is simple and rapid,highly sensitive and repeatable,and it could be used for the quantitative analysis of baicalin preparations.