材料科学
拉曼散射
纳米技术
拉曼光谱
腺苷
生物标志物
检出限
分析物
色谱法
化学
生物化学
光学
物理
作者
Tianxi Yang,Xiaoyu Guo,Yiping Wu,Hui Wang,Shuyue Fu,Ying Wen,Haifeng Yang
摘要
Adenosine plays a crucial role in the regulation of physiological activity in various tissues and organs. As adenosine is a possible biomarker for cancer, the determination of its level presents a demanding task for deeply monitoring progress of diseases. Through the synthesis of Fe3O4/Au/Ag nanocomposites weaved and stabilized by phytic acid and its salt, we develop a magnetically assisted surface-enhanced Raman scattering (SERS) protocol to determine trace level adenosine in urine samples from both lung cancer patients and health human. The magnetic properties of the nanocomposites enable to realize the simple separation of targeted molecules from a complex matrix and the Au/Ag nanoparticles moieties act as the SERS platform. This label-free Fe3O4/Au/Ag-nanocomposites-based SERS protocol shows a good stability, reproducibility, time efficiency (less than 20 min for one sample test), and huge sensitivity down to 1 × 10(-10) M. The protocol also has high selectivity because SERS signal of adenosine provides the molecular fingerprint information as well as an azo coupling pretreatment is performed to remove the interference of urea. Furthermore, a SERS array is designed for on-site screening adenosine in urine samples in a massive way using a portable Raman. Such a magnetically assisted SERS method as a powerful alternative can be expected as a smart and promising tool for effective assessment of healthcare.
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