An experimental study on therapeutic effect of Octreotide on lung injury in rat model of acute necrotizing pancreatitis

Objective To explore therapeutic effect of Octreotide on rat model of acute necrotizing pancreatitis(ANP) combined with acute lung injury(ALI).Methods Eighteen 21-day-old Sprague Dawley rats were randomly divided into three groups:control group(group C,n=6),treatment group(group T,n=6),experiment group(group E,n=6).ANP model was established by injecting 5% sodium taurocholate solution into bilo-pancreatic duct.After being anesthetized successfully,SD rats of group E were induced by injecting 5% sodium taurocholate solution into bilo-pancreatic duct,while those of group C were injected with 0.9% saline solution,but in group T,Octriotide had been used 1 hour before injecting 5%Taurocho1ic acid.Additionally for the group T,Octriotide was continuously given by a mini-pump via a central intravenous line.Arterial blood gas and serum amylase level were determined at the time point of 0,2,4,6?h.Bronchoalveolar 1avage fluid(BALF) was collected for analysis of neutrophil,polymorphnuclear leukocyte(PMN),macrophage(MA) and albumin level.Meanwhile,wet lung/dry lung weight ratio was counted.Results Arterial blood gas analysis indicated that PaO_2 of Group E increased at 2?h but decreased at 4?h and more significantly at 6?h;that Group C had no significant change;and that Group T had no significant difference with Group C but both Group C and Group T showed higher PaO_2 level as compared with Group E,suggesting the therapeutic effect of Octreotide.For all the three groups,serum amylase showed similar change with that of PaO_2 value.BALF parameters(PMN,MA and albumin level) were significantly higher in group E than those in group T and C.Conclusion Octreotide can significantly relieve alveolar inflammatory exudation in rat model of acute necrotizing pancreatitis associated with acute lung injury.