[Objective]The aim was to determine the best transplanting time of flue-cured tobacco in Lancang County. [Method]Three transplanting time,April 20,30 and May 10,was designed,and then investigated their growth,development and yield. [Result]Transplanting in April 20 had the longest growth period,was in favor of tobacco root growth,promoted the growth of overground parts of tobacco plants,had the better agronomic traits,promoted the synthesis of tobacco internal compounds,and then yield improved. Otherwise,transplanting in April 20 had stronger tobacco plants because of longer growth period,so the disease was relative lighter; the mature time of transplanting in April 20 was earlier because tobacco plants was transplanted earlier,so they avoided parts of rain weather at later growth period to decrease the later stage diseases; the mosaic of transplanting in April 20 was less at the earlier stage,so brown spot was decreased effectively at the later stage. [Conclusion]In the suitable time,early transplanting improves yield and internal quality of tobacco leaves. The suitable transplanting time of flue-cured tobacco was April 20 in Lancang County.