Establishment and Observation of Nude Mouse Diabetes Mellitus Model Induced by Streptozotocin

Objective Establish a diabetic model in nude mice by intraperitoneal injection of streptozotocin and observe changes of blood glucose and pancreatic tissue.Methods Eighty nude mice were divided into four groups separately intraperitoneal injected by 0 mg/Kg,100 mg/Kg,150 mg/Kg,200 mg/Kg STZ.To evaluate the model establishment,the blood glucose of diabetic nude mice was detected with a portable glucose meter.Pancreatic pathological changes were observed under light microscope.Results In the group injected by 150 mg/Kg STZ,17 mice were found with high blood glucose after 1 week and kept high blood glucose over 16.7 mmol/L over 10 weeks.The evidences from optical microscope observation further confirmed pathological changes on pancreatic islets cells of diabetic models along with the diabetic symptoms(polydipsia,polyphagia,diuresis).Conclusion With the dose of 150 mg/Kg of STZ through once intraperitoneal injection,we can effectively induce nude mice models of diabetes mellitus,especially in a large number models and the study of tissue engineering.