OBJECTIVE To established an analysis method for the determination of the content of polysaccharides from polysaccharide of Kiwifruit.MEHTODS Use the method of phenol-sulfuric acid.RESULTS There was a good linear relationship in wavelength 490 nm,r=0.9997,linear rang is 0~0.1mg·mL-1,RSD=2.65%.CONCLUSION This method is repeatable,accurate and simple.It can be used as determination of the content of polysaccharide in the Kiwifruit polysaccharide injection.