Study on the Interaction mechanism between Curcumin and Bovine Serum Albumin

Objective: To investigate the mechanism of interaction between curcumin and bovine serum albumin(BSA).Methods: Fluorescence spectroscopy was used to investigate the interaction between curcumin and BSA on different temperature,The Linewearver-Burk formula was used to process the experiment data.The binging constant and number of binding site were obtained.Results: The fluorescence intensity of BSA was quenched by curcumin,the binding constant(Ka) was large and was less affected by temperature.Conclusions: The mechanism of interaction between curcumin and BSA was a static quenching process.