Study on polyvinyl alcohol as entrapping agents for immobilizing the desulfurizing bacteria Pseudomonas stutzeri UP-2

The condition of immobilization operation of Pseudomonas stutzeri UP-2 and the condition of application of immobilized cell were inspected, using Pseudomonas stutzeri UP-2 possessing desulfurizing capabilities as the immobilization object, and dibenzothiophene (DBT) as a biocatalytic desulfurization model compound. The immobilized cell small globule of very good desulfurization function could be obtained when the concentration of PVA carrier was 10%, concentration of additive SA was 0.2%, the liquid bacteria ratio was 20:1 after 24h crosslinking in saturated solution of boracic acid containing 1% CaCl2 at 4℃. The reaction of 6d in the system of pH value 7.0 of 30℃ could degrade about 70% of DBT of the concentration of 576mg/L. The specific activity of the immobilized degrading DBT increased from 0.49mmol/gdw to 6.39mmol/gdw, with the application life raised reaching above 800h.