Study on FGF10,FGF18and FGFR3 Differential Expression in Hair Follicle of Aohan Fine-wool Sheep

The objectives of the present study were to identify the FGFs mRNA and protein differentially expression at different stages of hair follicle growth and development,and also to clarify the relationship between them.Firstly,gene chips were used to screen the differentially expressed genes in hair follicle at different stages and parts,FGF10,FGF18 and FGFR3,belonging to FGFs family,were identified.The qRT-PCR were used to verify the accuracy of gene chips results.Then by two-dimensional gel electrophoresis(2-DE)and mass-spectrometric technique(MS)technologies,differentially expressed proteins were identified,including FGF10,FGF18 and FGFR3proteins.The results showed FGF10 mRNA expression level was higher in abdomen than that inneck,suggested that it might play the negative regulation role in hair follicle development,and the expression of FGF10 protein was adverse.FGF18 mRNA expression level in abdomen was higher at anagen stage and protein expression was lower in neck,suggested it might inhibit the growth of hair follicles.FGFR3 had no consistent rules.These results showed that the expression of the 3genes at mRNA and protein levels are different in hair follicle at different stages and parts,which indicate the 3genes are related to the growth and development of hair follicle.The results may provide basic theory for understanding molecular mechanisms of FGFs regulating hair follicle growth and development,and also provide candidate genes for fine-wool breeding project.