[Differentially expressed genes in hepatocellular carcinoma of tree shrew induced by different factors].

肝细胞癌 肝硬化 乙型肝炎病毒 生物 基因 黄曲霉毒素 肝癌 微阵列 基因表达 病理 胃肠病学 内科学 医学 癌症研究 病毒 病毒学 遗传学 食品科学
作者
Li Y,Su Jj,Ji Cao,Chao Ou,Qiu Xk,Chia-Hsin Yang,Ban Kc,Yue Hf,Wei Wei,Ou Sj,Zhang Ls,Wan Df,Xianfeng Gu
出处
期刊:PubMed 卷期号:22 (10): 1018-22 被引量:1
链接
标识
摘要

Previous studies on differentially expressed genes in hepatocellular carcinoma (HCC) used to perform with para-cancerous tissues as normal control. However, the para-cancerous tissue of HCC is actually abnormal because they frequently contain hepatitis, cirrhosis, hyperplastic nodules or foci, etc. In order to explore the molecular mechanism and the responsible genes for hepatocarcinogenesis, through applying the HCC model of tree shrew (Tupaia belangeri chinensis), this study was designed to compare gene expression levels between HCC induced by different factors and their corresponding biopsies taken before HCC formation.Tree shrews were divided into two groups. Group AFB(1) was fed with aflatoxin B1 (AFB(1)). Group AFB(1)+HBV was infected firstly with human hepatitis B virus (HBV) and then fed with AFB(1) as group AFB(1). Serum tests for HBV markers and liver biopsies were performed periodically during the experiment. After appearance of HCC, 2 HCC samples from each group and their corresponding 30th-week biopsies were tested respectively by cDNA microarray assay. The gene expression levels were compared between each HCC and the corresponding biopsies, and the differentially expressed genes from the two groups of HCC induced by different factors were analyzed.The incidence rates of HCC in group AFB(1) and group AFB(1)+HBV were 73.3% and 77.8%, respectively. A considerable number of genes in both groups showed changes in their expression levels, which were mainly up-regulated in group AFB(1) but down-regulated in group AFB(1)+HBV. On the other hand, among the 588 checked genes (16 functional classifications) that were known related to human cancer, 11 genes were similarly expressed in all of the 4 HCC from the two animal groups. Most of these 11 genes belonged functionally to 3 types, namely "apoptosis-associated protein","DNA synthesis,repair and recombination proteins", and "growth factors, cytokines and chemokines".(1)HBV can affect AFB(1)-induced gene expression in certain extent. (2)The gene expression profiles of HCC induced by different factors are different. (3)The common differentially expressed genes in these two HCC groups are worthwhile for further study as the possibly responsible genes for hepatocarcinogenesis.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
曾建完成签到 ,获得积分10
刚刚
八乙基环辛四烯完成签到,获得积分10
10秒前
10秒前
benben应助科研通管家采纳,获得10
11秒前
猫沫沫829发布了新的文献求助10
16秒前
matilda完成签到 ,获得积分10
19秒前
小白完成签到 ,获得积分10
24秒前
ANT完成签到 ,获得积分10
26秒前
AHA完成签到,获得积分10
30秒前
34秒前
思源应助juphen2采纳,获得10
34秒前
35秒前
娜娜子完成签到 ,获得积分10
38秒前
39秒前
AthenaWang完成签到 ,获得积分10
45秒前
十二完成签到,获得积分10
48秒前
xgx984完成签到,获得积分10
49秒前
欢喜的慕青完成签到 ,获得积分10
54秒前
蔡从安完成签到,获得积分20
58秒前
科里斯皮尔应助蔡从安采纳,获得20
1分钟前
星辰大海应助蔡从安采纳,获得10
1分钟前
绿袖子完成签到,获得积分10
1分钟前
北辰完成签到 ,获得积分10
1分钟前
细心的雅绿完成签到 ,获得积分10
1分钟前
虚幻元风完成签到 ,获得积分10
1分钟前
踏实的无敌完成签到,获得积分10
1分钟前
1分钟前
zjw完成签到,获得积分10
1分钟前
几米杨发布了新的文献求助10
1分钟前
文艺水风完成签到 ,获得积分10
1分钟前
倩倩完成签到,获得积分10
1分钟前
Autin完成签到,获得积分10
1分钟前
小二郎应助几米杨采纳,获得10
1分钟前
黑粉头头完成签到,获得积分10
1分钟前
吴大宝完成签到,获得积分10
1分钟前
aq22完成签到 ,获得积分10
1分钟前
e394282438完成签到,获得积分10
1分钟前
bckl888完成签到,获得积分10
1分钟前
1分钟前
CGBY完成签到 ,获得积分10
1分钟前
高分求助中
请在求助之前详细阅读求助说明!!!! 20000
Sphäroguß als Werkstoff für Behälter zur Beförderung, Zwischen- und Endlagerung radioaktiver Stoffe - Untersuchung zu alternativen Eignungsnachweisen: Zusammenfassender Abschlußbericht 1500
One Man Talking: Selected Essays of Shao Xunmei, 1929–1939 1000
Yuwu Song, Biographical Dictionary of the People's Republic of China 700
[Lambert-Eaton syndrome without calcium channel autoantibodies] 520
The Three Stars Each: The Astrolabes and Related Texts 500
A radiographic standard of reference for the growing knee 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2468888
求助须知:如何正确求助?哪些是违规求助? 2136196
关于积分的说明 5442921
捐赠科研通 1860798
什么是DOI,文献DOI怎么找? 925473
版权声明 562694
科研通“疑难数据库(出版商)”最低求助积分说明 495093