Observation on the effect of high concentration of serum triglyceride on the detection of high density ipoprotein cholesterol by various mehtods

Objective To investiate the effect of different concentrations of serum triglyceride (TG) on the determination of high-density lipoprotein cholesterol (HDL-C) and look for a mehtods suitable for determinatio nof HDL-C at grass-roots laboratory. Methods TG、HDL-C in serum were determined by autobiochemical analyzer, and dilute serum specimen of high concentrations triglyceride specimen, and HDL-C was further detected by the methods of direct and precipitation, and the results of HDL-C were compared. Results There no difference was observed in determination of HDL-C between the 2 groups and the concentrations of serum TG was in the ranges of 1.69～4.00mmol/L. there significant difference was observed in determination of HDL-C as TG concentration was higher than 4.0mmol/L (P0.05). In determiantion of HDL-C befor and afer dilution of serum specimen significant differences were noticed as TG concentrations were higher than 6.00mmol/L; when HDL-C of diluted serum specimen was determined by identical methods, there was no difference among all with the methods of direct, there was difference as TG was higher than 4.01mmol/L with precipitation. Conclusion Significant differences in indetermination of concentration ofHDL-C with high TG level by precipitation and directly automatic methods have been observed,suggesting that HDL-C in the sera with high TG level should be determined by catalase HDL-C assay of directly automatic determination method.