Macrophage TRIM21 knockout inhibits septic acute lung injury by downregulating autophagy regulator protein ubiquitination

; BALF: bronchoalveolar lavage fluid; Bbox: B-box; BMDM: bone marrow-derived macrophages; CC: coiled-coil; CCL2/MCP1: C-C motif chemokine ligand 2; CHX: cycloheximide; CLP: cecum ligation puncture; co-IP: co-immunoprecipitation; CQ: chloroquine; DEG: differentially expressed genes; ELISA: enzyme-linked immunosorbent assay; GO: gene ontology; HE: hematoxylin and eosin; IL1B/IL-1β: interleukin 1 beta; KEGG: Kyoto Encyclopedia of Genes and Genomes; LPS: lipopolysaccharide; 3-MA: 3-methyladenine; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MRC1/CD206: mannose receptor, C type 1; NLRP3: NLR family, pyrin domain containing 3; NOS2/iNOS: nitric oxide synthase 2, inducible; PYCARD/ASC: PYD and CARD domain containing; RNA-seq: RNA-sequencing; RT-qPCR: reverse transcription quantitative PCR; TNF/TNF-α: tumor necrosis factor; ULK1: unc-51 like kinase 1.