Novel CoOOH-based fluorescent aptasensor for rapid and sensitive detection of sulfamethazine in environmental samples

Sulfamethazine (SMZ) has been widely used in animal husbandry and exposed to water and soil environments, posing potential threat to human health and ecological environment. Hence, we designed a CoOOH-based aptasensor, the fluorescence resonance energy transfer between FAM-labeled aptamer and CoOOH was used to sensitively and selectively detect SMZ in water and soil environments. Molecular docking and molecular dynamics simulations were used to predict binding mechanisms of SMZ and aptamer. Under optimized conditions, the aptasensor exhibited high sensitivity and selectivity with a linear range of 5-40 ng/mL and a limit of detection of 2.43 ng/mL. The recoveries of the aptasensor were 84.6-115.8% in water and soil samples with relative standard deviations below 9%, and the detection results were highly consistent with high-performance liquid chromatography. Therefore, this developed aptasensor was a reliable tool and could be applied to monitoring of SMZ in environmental samples.