检出限
抗坏血酸
碱性磷酸酶
牛血清白蛋白
化学
线性范围
生物传感器
钙钛矿(结构)
卤化物
荧光
血清白蛋白
色谱法
无机化学
核化学
生物化学
有机化学
酶
光学
物理
食品科学
作者
Lei Deng,Haibo Feng,Aomei Zhang,Tingting Wang,Minghui Yang,Xiaoqing Li,Xiang Chen
出处
期刊:Biosensors
[MDPI AG]
日期:2023-07-28
卷期号:13 (8): 770-770
被引量:1
摘要
Halide lead perovskite has attracted increased attention due to its excellent optical properties. However, the poor stability of the halide lead perovskite nanocrystals has been a major obstacle to their application in biosensing. Here, we proposed a method to synthesize CsPbBr3/BSA NCs perovskite using bovine serum albumin (BSA) as a zwitterion ligand. Then, a fluorescent sensor for alkaline phosphatase determination based on CsPbBr3/BSA NCs was successfully built via the interaction of L-ascorbic acid (AA) with BSA on the perovskite surface. Under optimal conditions, the sensor showed a linear concentration range from 50 to 500 μM with a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, and demonstrated a linear concentration range from 40 to 500 U/L with a detection limit of 15.5 U/L (signal-to-noise ratio of 3) for alkaline phosphatase (ALP). In addition, the proposed fluorescent biosensor exhibited good selectivity and recovery in the determination of ALP in human serum. This strategy offers an innovative way for enhancing the water stability of lead halide perovskite and promoting their application in biosensing areas.
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