数字聚合酶链反应
多路复用
多重聚合酶链反应
生物
呼吸系统
病毒学
呼吸道
实时聚合酶链反应
呼吸道感染
聚合酶链反应
微生物学
基因
生物信息学
遗传学
解剖
作者
Xiangpeng Chen,Fang Wang,Yuhong Fu,Lan Huang,Fĕi Li,Hongwei Zhao,Xiaolei Guan,Qiuping Li,Qi Li,Yilu Wang,Yong Guo,Zhengde Xie
出处
期刊:Virology
[Elsevier]
日期:2024-02-01
卷期号:590: 109948-109948
标识
DOI:10.1016/j.virol.2023.109948
摘要
The emergence of multiplex digital polymerase chain reaction (dPCR) and other detection technologies for respiratory pathogens in recent years has facilitated greater understanding of respiratory virus epidemics. In this study, a multiplex dPCR method was developed and evaluated as a means of detecting five respiratory pathogens in children with acute lower respiratory tract infection (ALRTI). With 139 nasopharyngeal swabs collected from children with ALRTI, pathogens were detected using dPCR and quantitative real-time PCR (qPCR) methods. Of those specimens, dPCR detected 86 positive cases, while qPCR identified 84. Moreover, dPCR exhibited higher sensitivity than qPCR, and displayed no cross-reactivity with common respiratory pathogens. These findings suggest that dPCR-based method could become one of the most promising options for acute respiratory pathogen detection.
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