C2C12型
基因敲除
生物
细胞生物学
肌发生
细胞生长
骨骼肌
心肌细胞
小RNA
下调和上调
细胞分化
钙粘蛋白
分子生物学
基因
遗传学
细胞
内分泌学
作者
Chengchuang Song,Qi Wang,Qi Qi,Xi Chen,Yanhong Wang,Chunlei Zhang,Fang Xintang
标识
DOI:10.1080/10495398.2022.2109042
摘要
MircoRNAs (miRNAs) play an important role in skeletal muscle development. Previous study had found that miR-495-3p was differentially expressed in fetal and adult goat skeletal muscle, but its function in myogenic proliferation and differentiation are unclear. Herein, we found the expression of miR-495-3p in C2C12 was downregulated during proliferation stage and upregulated during differentiation stage. Functionally, overexpression of miR-495-3p in C2C12 inhibited proliferation, and promoted myogenic differentiation. Mechanistically, the luciferase reporter assay demonstrated that cadherin 2 (CDH2) was a potential target gene of miR-495-3p. Importantly, overexpression of miR-495-3p inhibited CDH2 expression. Furthermore, knockdown of CDH2 in C2C12 inhibited proliferation and promoted myogenic differentiation. Together, the results showed that miR-495-3p inhibits C2C12 proliferation and promotes myogenic differentiation through targeting CDH2.
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