Determination of Effective Components of Scutellaria indica from Different Habitats

Abstract A HPLC method for the determination of three effective components in Scutellaria indica from different habitats was established. Analysis was performed on Waters Spherisorb ODS2 (4.6 mm × 250 mm, 5 μ m) with gradient mobile phase of 0.5% glacial acetic acid aqueous solution-acetonitrile. The flow rate was 1 m L/min and the detection wavelength was set at 275 nm. The results showed that the chromatographic elution curves with good resolution and reproducibility were obtained. By comparing with the reference materials, three effective components were determined, namely, scutellarin, scutellarin and apigenin. The method is sensitive, accurate and repeatable, and can be used for the determination of three components in Scutellaria indica. The results also showed that there were significant differences in the chemical composition of Scutellaria indica from different areas. The quality differences of Scutellaria indica from different areas should be considered in the selection of Scutellaria indica.