光防护
光合作用
拟南芥
生物
光系统II
叶绿素荧光
植物
光抑制
叶绿素
叶绿体
拟南芥
光合能力
叶黄素
光系统
光合效率
光强度
化学
非光化学猝灭
突变体
作者
Man Zhang,Jing Zhao,Wanying Li,Shuqi Wen,Huiling Huang,Jie Dong,Bing Liu,Gong Zhang,Hong-Bin Wang,Yanting Shen,Hong-Lei Jin
标识
DOI:10.1016/j.envexpbot.2020.104350
摘要
Abstract The Qinghai-Tibet Plateau (QTP) is the highest and largest young plateau in the world, with a wide variety of extreme environments. Due to the low density of air and high transparency of the atmosphere, QTP has abundant sunshine and high light intensity. It is important for plants in QTP to evolve mechanism of photoprotection to adapt the high-light conditions. Arabidopsis thaliana is an important model organism in plant science and molecular biology. Tibet-0 was the first Arabidopsis thaliana ecotype collected in the QTP, at >4000 m above sea level. Here, we systematically analyzed the photosynthetic responses of Tibet-0 to high light. Our results show that Tibet-0 has more efficient PSII photosynthetic activity under continuous high-light conditions (1000 μmol photons m−2 s−1) compared to Columbia (Col-0). Chlorophyll fluorescence analysis showed that Tibet-0 exhibited enhanced PSII quantum yield (ФPSII), photochemical quenching (qP), and electron transport rate (ETR) under high-light conditions. However, anthocyanin accumulation was lower in Tibet-0. Blue Native-PAGE and immunoblot analyses showed that the abundances of PSII complexes and subunits were significantly higher in Tibet-0 vs. Col-0 under standard and high-light conditions. Notably, the associations of PSII core subunit mRNAs psbA, psbB, psbC and psbD with chloroplast ribosomal protein were significantly higher in Tibet-0, based on ribosome-nascent chain complex (RNC)-quantitative PCR. Moreover, the expression of amounts of chloroplast ribosomal protein-related genes was activated in Tibet-0 after high-light treatment. These results suggest that the enhanced photoprotection capability of Tibet-0 arises via constitutively higher translation efficiency for PSII proteins due to activated ribosomal protein expression. This study thus provides insight into the mechanism underlying high-light adaptation in plants at high altitudes.
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