Monokaryons isolated from single spores of 29 Flammulina velutipes strains were mated with each other and the formation of dikaryons determined by the presence of clamp connections.Ten A and eight B mating factors were identified.Mating types of 13 yellow strains consisted of different combinations of A1 to A9 and B1 to B8 mating factors,whereas the majority of white strains were of the A2A3B2B3 mating type.The four mating types observed among the monokaryons derived from 24 strains were present in a 1∶1∶1∶1 ratio whereas mating types in monokaryons derived from the remaining strains deviated from the theoretical distribution.