Regulation and Homologous Expression of PNA in Panax ginseng by RNAI

Panax ginseng (C.A.Meyer), belonging to Araliaceae family Panax category, has important commercial value and are used in worldwide. Ginsenosides, the major bioactive ingredients of P. ginseng, are classified into two groups by the skeleton of aglycones, namely dammarane-type and oleanane-type. Dammarane-type ginsenosides dominate over oleanane-type not only in amount but also in structural varieties. Here, we cloned and identified the Dammarenediol-II synthase gene named PNA encoding encoding enzymes catalyzed 2,3-oxidosqualene to dammarendediol-II by RT-PCT.we established PNA RNAi transgenic. The RT-PCT and HPLC analysis of the final products of protopanaxadiol-type and protopanaxatrio-type ginsenosides showed a result that declined level of protopanaxadiol-type and protoanaxatriol-type ginsenosides, and increased phytosterol content.