生物合成
内酰胺
化学
甲基转移酶
甲基化
酶
生物化学
立体化学
O-甲基转移酶
乙酰化
蛋氨酸
转移酶
辅因子
基因
氨基酸
作者
Jiaqi Lyu,Richiro Ushimaru,Ikuro Abe
标识
DOI:10.1021/acs.orglett.2c00878
摘要
Tabtoxin is a β-lactam ring-containing phytotoxin produced by a plant pathogenic Pseudomonas species. Here, we describe the early stages of tabtoxin biosynthesis, involving a C-methylation reaction catalyzed by the S-adenosyl-l-methionine-dependent methyltransferase TblA as the initial step for the β-lactam construction. Gene deletion and in vitro biochemical assays demonstrated that the Gcn5-related N-acetyltransferase domain of TblD catalyzes the acetylation of the α-amino group of 5-methyl-l-lysine. This establishment of the early reaction steps lays the foundation for characterizing unique β-lactam biosynthesis.
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