白色念珠菌
白色体
细胞凋亡
膜联蛋白
细胞色素c
微生物学
碎片(计算)
半胱氨酸蛋白酶3
磷脂酰丝氨酸
生物
标记法
化学
程序性细胞死亡
生物化学
磷脂
膜
生态学
作者
Chong Pang,Jianshuang Chen,Shuangyan Liu,Hao Miao
出处
期刊:Research Square - Research Square
日期:2022-06-27
标识
DOI:10.21203/rs.3.rs-1733318/v1
摘要
Abstract Our previous studies showed that Shikonin (SK) had a strong anti- Candida albicans ( C. albicans ) activity, especially against some fluconazole-resistant strains, which is probably due to the oxidative damage of SK to C. albicans . In this study, we further analyze the exact mechanism of SK against C. albicans . Through some experiments, such as detecting the apoptosis and necrosis rates of C. albicans SC5314 cells treated with different concentrations of SK by Annexin V-FITC / PI double staining, observing the ultrastructure of cells by transmission electron microscopy, testing mitochondrial cytochrome c oxidase activity, caspase activity, and caspase-related gene CaMCA1 expression level. We found that SK could induce a series of apoptosis characteristics, including phosphatidylserine (PS) externalization, chromatin condensation and fragmentation, decreased cytochrome c oxidase activity as well as caspase activation. In summary, this study highlighted the antifungal activity and mechanism of SK against C. albicans , providing a potential therapeutic strategy for C. albicans infection.
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