合胞滋养细胞
污渍
免疫印迹
生物
缺氧(环境)
胎盘
过氧化物酶体增殖物激活受体
免疫组织化学
北方斑点
实时聚合酶链反应
内分泌学
内科学
受体
基因表达
男科
胎儿
化学
基因
免疫学
医学
生物化学
怀孕
有机化学
氧气
遗传学
作者
Ting‐Ting Chang,Ming–Kwang Shyu,Min-Chuan Huang,Chen-Chi Hsu,Szu-Yu Yeh,Mei‐Ru Chen,Chun-Jung Lin
摘要
The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPARα. OCTN2 and PPARα expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical (IHC) analysis, Western blotting, and quantitative polymerase chain reaction (qPCR). Effects of hypoxia on the expression of OCTN2 and PPARα in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPARα protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPARα expression in human placental explants and in BeWo cells. WY14643, a PPARα agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPARα-mediated pathways.
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