Size‐Controlled Facile Synthesis of Silver Nanoparticles by Chemical Reduction Method and Analysis of Their Antibacterial Performance

Abstract This article aimed at the size‐controlled one‐pot facile synthesis of silver nanoparticles (AgNPs) by chemical reduction method and their antibacterial response against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. AgNPs coated with and without trisodium citrate (TSC) were synthesized using silver nitrate as a precursor and hydrazine as a reducing agent. Spherical AgNPs (average size 35 nm) formation, capping agents attachment, and product purity was confirmed by ultraviolet‐visible (UV–vis) spectroscopy, X‐ray diffraction (XRD), field emission scanning microscopy (FESEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), the thermogravimetric analysis (TGA), and energy‐dispersive X‐ray (EDX) spectroscopy. Exposing the synthesized TSC uncoated and coated AgNPs to Gram‐negative E. coli bacteria at a concentration of 50 mg L −1 , the zone inhibition was found to be 5 mm and 6 mm, respectively. Again, TSC coated AgNPs exhibited a notable zone inhibition of 8 mm to Gram‐positive S. aureus . These results suggest that chemically synthesized TSC coated AgNPs could be used as an antibiotic against S. aureus and E. coli , which is very inspiring.