阿克曼西亚
偶氮甲烷
某种肠道细菌
普氏粪杆菌
医学
结直肠癌
肠道菌群
双歧杆菌
短双歧杆菌
癌症
药理学
乳酸菌
癌症研究
内科学
免疫学
化学
生物化学
发酵
作者
Yishan Wang,Xiaodi Zhang,Jiawei Li,Ying Zhang,Yingjie Guo,Qing Chang,Li Chen,Yiwei Wang,Siyao Wang,You-Jin Song,Yongkun Zhao,Zhihong Wang
标识
DOI:10.3389/fphar.2021.609992
摘要
Sini Decoction (SND), as a classic prescription of Traditional Chinese Medicine (TCM), has been proved to be clinically useful in cardiomyopathy and inflammatory bowel diseases. However, the role and mechanism of SND in colitis-associated cancer remains unclear. This study aims to evaluate the effect of SND on colorectal cancer(CRC) symptoms and further explore the changes of gut microbes mediated by SND extract in azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced CRC mice through 16 S rRNA sequencing. Our results indicated that treatment with SND extract could ameliorate the tumors' malignant degree by decreasing tumor number and size. Also, the expression levels of Cyclooxygenase 2 and Mucin-2, which are typical CRC biomarkers, were reduced compared to the CRC group. In the meantime, SND extract can upregulate CD8 + T lymphocytes' expression and Occludin in the colonic mucosal layer. Besides, SND inhibited the expression of CD4 + T cells and inflammatory cytokines in CRC tissue. According to bioinformatics analysis, SND extract was also suggested could modulate the gut microbial community. After the SND treatment, compared with the CRC mice model, the number of pathogenic bacteria showed a significant reduction, including Bacteroides fragilis and Sulphate-reducing bacteria ; and SND increased the relative contents of the beneficial bacteria, including Lactobacillus, Bacillus coagulans , Akkermansia muciniphila, and Bifidobacterium . In summary, SND can effectively intervene in colorectal cancer development by regulating intestinal immunity, protecting the colonic mucosal barrier, and SND can change the intestinal microbiota composition in mice.
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