Identification and Isolation of Mice and Human Hematopoietic Stem Cells

Hematopoietic stem cells (HSCs) are multipotent cells capable of differentiating into all types of blood cells. The important feature of the HSCs is their ability to repopulate the complete blood cells after BM ablation. For clinical application, cord blood derived HSCs and G-CSF mobilized peripheral blood HSCs are good alternative to bone marrow HSCs. For immunological and hematological studies the obvious choice of model organism is Mouse. Therefore, understanding HSCs in murine model is important. In this chapter, we describe the common/currently used methods to isolate and identify human and mouse HSCs.