碱性磷酸酶
干细胞
脂肪组织
染色
川地34
胶原酶
油红O
分子生物学
茜素红
化学
生物
脂肪生成
细胞生物学
生物化学
遗传学
酶
作者
Yu Ren,Haiqing Wang,Yuzhen Ma,Ming Cang,Rui Wang,Dongjun Liu
出处
期刊:PubMed
日期:2010-12-01
卷期号:26 (12): 1645-51
被引量:4
摘要
To obtain bovine adipose-derived stem cells (ADSCs), bovine ADSCs were digested in collagenase type I solution. The growth curve of ADSCs was checked by cell counting. Chromosome analysis was checked. The molecular markers of ADSCs were detected with immunofluorescence staining. The morphology of ADSCs was identical to fibroblast like and the cells showed active proliferative ability. Vimentin, CD49d and CD13 antigens were detected, but CD34 antigen was negative. Alkaline phosphatase activity was greater in ADSCs during calcification, and Alizarin Red staining was positive. Lipid droplets were apparent around cells during adipogenesis, and Oil Red-O staining was positive. The results demonstrated that ADSCs could be used as seed cells for tissue engineering due to the simple isolation, differentiation and stable and active growth.
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