Protective effect of piperphentonamine on global cerebral ischemia/reperfusion injury in rats

Objective:To investigate the effect of piperphentonamine(PPTA) on global cerebral ischemia/reperfusion(I/R) injury in rats.Methods: Global I/R injury was induced by four-vessel occlusion in rats.Then the rats were randomly divided into control,I/R,and PPTA(2.5,5 and 10mg·kg-1) groups.Morris water maze was used to evaluate the learning and memory abilities.The histological changes in the hippocampus were observed after Nissl staining.LDH activity and the expression of bax/bcl-2,caspase-3 and iNOS mRNA were measured by colorimetric assay kit and real-time PCR.Results: PPTA(5 and 10mg·kg-1) significantly shortened escape latency in the Morris water maze test,and protected neurons from injury.The LDH activity and the expression of bax/bcl-2,caspase-3 and iNOS were reduced compared with I/R group.Conclusion: PPTA can reduce neuronal damage in the hippocampus,and enhance the ability of learning and memory after global I/R injury in rats.