A Comparative Study on Two Staining Methods of Nucleic Acid

Objective: To compare the effect and their sensitivity of two different staining methods to DNA in the gels, and evaluate the possible application of SYBR GreenⅠin routine DNA staining as a substitute for ethidium bromide(EB). Methods: The new nucleic acid staining SYBR GreenⅠand EB were used to stain the DNA in the gel, perfectively stain before electrophoresis and after electrophoresis. Results: There was no distinct difference between the two methods. Both SYBR GreenⅠand EB stain could detect as little as 10 ng per band of DNA. Conclusion: The effect and sensitivity of the two staining methods are no distinct difference, and SYBR GreenⅠand EB may be used in these staining methods. SYBR GreenⅠ, which is a new nucleic acid stain may substitute for EB as a general dyestuff to stain the DNA in the gel.