Preparation and Characterization of Collagen/dialdehyde Pullulan Complex Scaffold Materials

Collagen scaffolds have been promising to provide suitable environment for 3D cell culture in tissues engineering.However,its rapid degradation and week mechanical properties remained problems that limited many of its applications.In this study,collagen/dialdehyde pullulan complex scaffolds were prepared by mixing collagen with dialdehyde pullulan with different aldehyde contents to chemically cross-link the collagen through the formation of Schiff's base between the amino on collagen and the aldehyde on pullulan.With higher aldehyde content in pullulan,the complex scaffold thus formed showed improved degradation resistance.The MTT assay indicates that the cell proliferation on the complex scaffolds had not different with that on the pure collagen scaffold.These complex scaffolds are suitable for 3D cell culture in tissue engineering.