Analysis of paralytic shellfish poisoning in shellfish of Dalian coast

The economic shellfish in Dalian coast were collected from Jan.2007 to Oct.2008.The paralytic shellfish poisoning(PSP)in shellfish was determined by the mouse bioassay.The results showed that the high values of PSP of economic shellfish were found from Apr.to Sep.of 2007 and from Feb.to Aug.of 2008 in Dalian coast.The high values of PSP were found in Caishen Island of Changhai and Hei Island of Zhuanghe.The major findings of PSP were in scallop Patinopecten yessoensis,not in clams Scrobicularia plana and oyster Crassostrea gigas.The paralytic shellfish poison-binding proteins(PSPBP) in different tissues were studied by ELISA and the result showed that the proteins in muscle strongly bind with PSP.PSPBP were isolated by DEAE 52 and Sephadex G-100.SDS-PAGE showed that the PSPBP has a molecular mass of 113 kDa and 72.4 kDa.