Determination of Molecular Weight and Analysis of Monosaccharide Composition in Isolation of Polysaccharide from Imperata Cylindrica

Objective:To study on isolation the polysaccharide from Imperata cylindrica and to establish a method for the determination of the molecular weight of the polysaccharides in Imperata cylindrica,and to measure its composition and mole ratio through HPLC.Method:The polysaccharide in Imperata cylindrica was Isolated and purified through the way of the staged alcohol deposition.Dextran was used as standard sample to draw the molecular weight standard curve,and the molecular weight of Imperata cylindrica polysaccharides was determined by HPLC,the polysaccharide was hydroyzed by acid to analyze monosaccharide composition and the mole ratio by HPLC.Result:The molecular weight of Imperata cylindrica polysaccharide was 8 292.2.According to HPLC IC1 was composed of Rhamnose,Xylose,Fructose,Mannose and Glucose,the mole ratio of these monosaccharides was 1∶11.45∶1.26∶1.02∶59.23.Conclusion:This method is simple accurate and sensitive.