Determination of extracellular fibrinolytic activity of nattokinase by ultraviolet spectrophotometry

To establish a quantitative method for the determination of extracellular fibrinolytic activity of nattokinase in a short time.Prepare blood fibrin for extracellular reaction system,and then add nattokinase sample to the system for fibrinolytic reaction.Determine the absorbance of the soluble hydrolysate produced by nattokinase by ultraviolet spectrophotometry.Thereby,determine the fibrinolytic activity of nattokinase and evaluate the feasibility of the method.The method was sensitive,accurate,precise,and fast.This method could be used for the quantitative analysis of extracellular fibrinolytic activity of nattokinase.