Solution and Purification of ECRG2 Recombinant Fusion Protein Inclusion Body

In this study,ORF of ECRG2 cDNA was cloned into the pEGX-4T-1 vector and expressed in Escherichia coli as a GST fusion protein.Inclusion body GST-ECRG2 was collected,dissolved in SKL,refolded in PBS and purified using GST affinity column.After optimization,purified protein has been obtained at high yield.