Culture of Complex Denitrification Sludge and Its Denitrification Properties

With the purpose to solve the problems in the bio-denitrification process,a strain with denitrification ability was separated and identified as Pseudomonas stutzeri. Complex denitrification sludge was cultured by the coupling growth of the strain and bio-active carbon sludge,and the denitrification properties were studied. Results showed that the microphoto shape of the complex denitrification sludge was granular irregularly with no filamentous bacteria. SVI was 38~55mL/g and VSS was 2.0g/L. As for the different denitrification substrates such as NO3--N and NO2--N,the denitrification curves were similar. There was substrates competitive relationship between NO3--N and NO2--N when they coexisted in the same simulated wastewater,but no inhibitive effect occured by NO2--N. When DO was below 0.5mg/L,the denitrification effect was good,and in the high pH of 9.3,denitrification could take place well. The volume loading could get to 0.9kgNO3--N(/m·3d).