Preparation of Acrylamide Using Resting Cells

The resting cells were used to produce acrylamide from acrylonitrile by enzyme hydration in semi-continuous process. The result indicated that the enzyme Nocardia sp was deactivated at temperature more than 20 ℃, it was also intensely inhibited when the volume fraction of acrylonitrile was above 4% and product concentration above 30%. The enzyme activity could be kept in considerable level when acrylonitrile and enzyme were added in stream-form and at low temperature. When volume fraction of enzyme was 10% and at 20 ℃ by this addition mode acrylamide could be obtained in mass concentration of 23% more than that by batch-mode addition process.