Establishment of a mouse model of pulmonary adenocarcinoma

Objective To establish a model of pulmonary adenocarcinoma in mice,and to supply an experiment platform for screen of important miRNAs in the process of pulmonary adenocarcinoma. Methods The pulmonary adenocarcinoma model was established by intranasal administration of adenovirus Cre to Lox-stop-lox K-ras G12D mice.The lung tissues of model mice were digested with dispase and collagenase Ⅳ in combination,and then the expression of Sca-1 and CD34 was detected by the dual-color immunofluorescence. Results The model of pulmonary adenocarcinoma was established successfully,and Sca-1 and CD34 were expressed in the lung of model mice. Conclusion The model of pulmonary adenocarcinoma can be established successfully for further study on screen of important miRNAs in the process of pulmonary adenocarcinoma.