细胞凋亡
蛋白激酶B
PI3K/AKT/mTOR通路
癌症研究
黑色素瘤
PTEN公司
体内
异鼠李素
化学
细胞生长
分子生物学
医学
生物
生物化学
槲皮素
抗氧化剂
遗传学
山奈酚
作者
Ran Duan,Xiao Liang,Bangda Chai,Yiwen Zhou,Hengyu Du,Yingjun Suo,Zhaohuan Chen,Qingfeng Li,Xiaolu Huang
摘要
Malignant melanoma is characterized by its bad prognosis for aggressiveness, drug resistance, and early metastasis. Isorhamnetin (3′-methoxy-3,4′,5,7-tetrahydroxyflavone; IH) is a natural flavonoid that has been investigated for its antitumor effects in breast cancer, colon cancer, and gastric cancer through inducing cell apoptosis. Given its role in tumor inhibition, no research has been conducted concerning its effect against melanoma. In the present study, we found that IH could significantly inhibit B16F10 cell proliferation and migration and induce B16F10 cell apoptosis. The examination on molecular mechanism revealed that IH could suppress the phosphorylation of Akt and the translocation of NF- κ B, which are key factors in apoptosis-related pathways. We also detected that this process was related to the bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases 4 (PFKFB4) by PFKFB4 knockdown experiment. In line with in vitro study, we further provided that IH effectively inhibited tumor growth in vivo. Taken together, IH was proven to induce melanoma cell apoptosis in vitro and in vivo, which may serve as a potential agent in malignant melanoma treatment in the future.
科研通智能强力驱动
Strongly Powered by AbleSci AI