氢-氘交换
化学
质谱法
稳健性(进化)
生物制药
氘
生化工程
纳米技术
色谱法
物理
材料科学
工程类
基因
生物
量子力学
生物化学
遗传学
作者
Ellie I. James,Taylor A. Murphree,Clint Vorauer,John R. Engen,Miklós Guttman
出处
期刊:Chemical Reviews
[American Chemical Society]
日期:2021-09-07
卷期号:122 (8): 7562-7623
被引量:102
标识
DOI:10.1021/acs.chemrev.1c00279
摘要
Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is a widespread tool for structural analysis across academia and the biopharmaceutical industry. By monitoring the exchangeability of backbone amide protons, HDX-MS can reveal information about higher-order structure and dynamics throughout a protein, can track protein folding pathways, map interaction sites, and assess conformational states of protein samples. The combination of the versatility of the hydrogen/deuterium exchange reaction with the sensitivity of mass spectrometry has enabled the study of extremely challenging protein systems, some of which cannot be suitably studied using other techniques. Improvements over the past three decades have continually increased throughput, robustness, and expanded the limits of what is feasible for HDX-MS investigations. To provide an overview for researchers seeking to utilize and derive the most from HDX-MS for protein structural analysis, we summarize the fundamental principles, basic methodology, strengths and weaknesses, and the established applications of HDX-MS while highlighting new developments and applications.
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