改性大气
食物腐败
食品科学
肉类腐败
16S核糖体RNA
肉类包装业
保质期
生物
放大器
生物技术
扩增子测序
温度梯度凝胶电泳
细菌
聚合酶链反应
生物化学
基因
遗传学
作者
Anthony Pius Bassey,Yongfang Chen,Zongshuai Zhu,Olumide A. Odeyemi,Evans Boateng Frimpong,Keping Ye,Chunbao Li
标识
DOI:10.1016/j.foodres.2021.110412
摘要
Modified atmosphere packaging (MAP) is widely applied in packaging meat and meat products. While most studies had employed culture-dependent microbiological analyses or polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), the recent application of high-throughput sequencing (HTS) has been effective and reliable in detecting the microbial consortium associated with food spoilage. Since MAP application is limited in China, applying HTS in assessing the microbial consortium of meat and meat products in the country becomes imperative. In this study, quality indexes and bacterial enumeration often used as spoilage indicators were employed to assess MAP fresh pork under chilled (4 °C) storage for 21 d. The results indicated that 70%O2/30%CO2 (Group A) retained more redness (a*) content, while 70%N2/30%CO2 (Group B) markedly reduced spoilage indicators compared to the control group. Notably, high-throughput sequencing indicated that Group B and 20%O2/60%N2/20%CO2 (Group C) inhibited the growth of abundant spoilers, Pseudomonas spp. and Brochothrix spp. Thus, MAP (Group B and C) has promising potential in inhibiting predominant meat spoilers during chilled storage. This study provides valuable information to food industries on the potential application of MAP to control meat spoilage in Chinese markets.
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