金黄色葡萄球菌
细菌
化学
检出限
微生物学
病菌
食品科学
重组酶聚合酶扩增
色谱法
生物
实时聚合酶链反应
生物化学
遗传学
基因
作者
Fangbin Xiao,Ju Liu,Xiaoyan Feng,Guoyang Xie,Wanqing Zhang,Hengyi Xu
标识
DOI:10.1016/j.microc.2022.107388
摘要
Staphylococcus aureus (S. aureus) is a common foodborne pathogen, which could cause serious bacteremia and infective endocarditis. Establishing a rapid and low-cost method to capture and detect S. aureus in food samples is necessary. In this study, we have developed a method on the basis of polyethyleneimine-functionalized magnetic nanoparticles (MNP-PEI) and recombinase aided amplification (RAA) for rapid enrichment and analysis of S. aureus in milk. The capture efficiency of MNP-PEI on multiple strains of bacteria and influencing factors were studied. The limits of detection (LOD) of the MNP-PEI-RAA strategy were 2.9 × 101 CFU·mL−1 in phosphate-buffered saline (PBS) and 2.9 × 102 CFU·mL−1 in milk. The entire enrichment and detection processes could be completed within 60 min. The detection strategy based on MNP-PEI and RAA had low cost, short detection time, and high sensitivity. In addition, the broad-spectrum of bacteria captured by MNP-PEI was important for simultaneous analysis of a variety of bacteria, which provided an effective tool for evaluating the microbiological safety of food samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI