Protein and polysaccharide-based asymmetric mat with tuned bilayer configuration for enhanced wound healing efficiency

In this research we focused on the fabrication of an asymmetric bilayer membrane with core-shell/simple layer configuration providing the functions of needed hierarchically hydrophilicity and porosity, anti-infectious, tissue adhesion as well as degradation and integration with tissue, cells proliferation, and enhanced promotion of tissue regeneration. The bilayer membrane composed of collagen (Col), chitosan (CS), aloe vera (AV) and gelatin (Gel), not only simulates the features of the epidermis and dermis layer of a natural skin but also benefits from the materials necessary for the regeneration of injured skin tissue during the healing process. The results of full-thickness skin wound evaluation revealed that the fabricated asymmetric membrane could facilitate wound healing within 10 days mainly through enhancing cellular activities, enhancing collagen deposition, and promoting proliferation. Results of histopathological analysis and immunohistochemistry after 10 days of treatment, demonstrated more re-epithelialization and collagen density for the treated groups compared to the control group.